Part:BBa_K2306014:Design

Efficient RBS from bacteriophage T7 gene 10 and Shine Dalgarno

Very strong ribosome binding site resulting from the combination of a gene 10 of bacteriophage T7 (g10-L) RBS and the Shine-Dalgarno RBS.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

The ribosome binding site derived from gene 10 of bacteriophage T7 (g10-L) causes a pronounced stimulation of expression when placed upstream of a variety of genes. However, if such a g10-L sequence is placed upstream of another RBS, as that of the Shine-Dalgarno sequence, a significant increase in the translation of the genes is observed.

Design Notes

This RBS was designed to achieve the highest translational efficiency for the production and characterization of proteins. The design of this RBS comes down to 5 bp, the g10-L and Shine-Dalgarno sequences, and 7bp extra downstream. Thus, it is ready to be included in your constructs after your promoter and before the start codon of your gene.

Source

Olins and Rangwala (1989);

References

Olins,P. O., Devine, C. S.,Rangwala,S.H., Kavka,K.S.(1988); Olins and Rangwala,(1989);